Computational resources

Binder production and applications generate large quantities of data. To realise its full value, a Europe-wide resource of binders must master these data and derive their full benefit. Data from binder users is an important resource thus far underexploited, and submission of data must be encouraged through the development of web-based tools that provide immediate benefit to users. The availability of objective binder evaluation data through public access databases and web portals will therefore bring a major long-term benefit to the research community, which has often struggled with uneven or unreliable information. Design of bioinformatics standards, tools and databases to support development of protein binders, started within ProteomeBinders by AFFINOMICS partners EMBL EBI and KTH, will be continued and enlarged. An important example is the community-based portal 'Proteinbinders', recently developed by partner KTH, a web resource which currently provides descriptive data on antibodies together with detailed quality control and opportunity for submission of user data and assessments. Proteinbinders will be expanded to include all affinity reagent types. Through the Human Proteome Resource Centre, KTH also provides the public Human Protein Atlas portal, a database with >2 million high-resolution images of protein distribution in normal and cancer tissues. The IntAct and MINT molecular interaction databases (EU Temblor, Felics, and Sling projects) will capture AFFINOMICS results on proteomics of cell signalling and cancer (generated in WP6) and present them in the context of existing public domain data; they will provide an integrated view of molecular interactions, complexes, pathways, and binders used/available for their characterisation, using the PSI-PAR data representation developed in the ProteomeBinders programme (EMBL EBI). AFFINOMICS protocols and SOPs will be centrally archived and disseminated through the MolMeth database (UU).