Uppsala University is one of the leading comprehensive research universities in Sweden. The University also has a particularly strong tradition in technologies for biological analyses.
The Centre for Advanced Molecular Analysis focuses on the development of molecular tools for high-precision, high-throughput analyses of genes and proteins. Headed by Prof Ulf Landegren, the Centre has pioneered a number of important molecular techniques, such as the oligonucleotide ligation assay, padlock probes, and more recently the proximity ligation technique. Several have been licensed for industrial and clinical applications. Prof Fredrik Pontén’s group focuses on antibody-based proteomics, profiling protein expression on a genome-wide scale using tissue and cell microarrays, as part of the ambitious Human Protein Atlas project (see also partner 2 KTH). Assoc Prof Erik Bongcam-Rudloff leads a group leading a COST Action: Next Generation Sequencing Data Analysis Network and a FP7 project: ALLBIO. The Bongcam-Rudloff group has created the bioinformatics suite eBioinformatics and several onlina analysis tools
Role in AFFINOMICS: UU contributes expertise in applying the proximity ligation methodology for highly sensitive protein binder-based detection of soluble proteins and proteins expressed in tissues (WP5), and detection of protein-protein interactions for functional analysis of signal transduction pathways (WP6) . UU also provides antibody-based protein profiling, including characterisation of the distribution of proteins and protein complexes detected by reagents produced in the project, taking advantage of their high-throughput facility for immunohistochemical staining, slide scanning facilities, and histopathology analysis. UU also further develop their Molecular Methods database (MolMeth) as a repository for methods used in affinity proteomics (WP7). Dr. Erik Bongcam-Rudloff is WP7 coordinator and Professor Ulf Landegren is WP6 coordinator in the Affinomics project.