University of Zurich
The Universität Zürich is one of Europe's foremost research universities with a particular strength in the life sciences. The Department of Biochemistry, with full access to the Functional Genomics Center, is well equipped to do this kind of research.
Professor Andreas Plückthun’s laboratory has made numerous seminal contributions to the field of antibody engineering over the last 20 years, beginning with the first functional expression in bacteria, biophysical characterisation and stabilisation, to development of a synthetic antibody library. His group has pioneered and developed several evolutionary technologies such as ribosome display. More recently, they developed a new set of binding molecules based on synthetic ankyrin repeat proteins (DARPins), which offer an extraordinary expression yield and thus a realistic prospect to obtain high-affinity binders in a very short time. The combination of strong biophysical chemistry with novel evolutionary technologies puts the lab in a strong position of developing new proteins.
Role in AFFINOMICS: Andreas Plückthun’s group is responsible for production of DARPins as a representative scaffold protein binder against kinases and other signal transduction targets in WP2. They also employ these molecules in mechanistic and intracellular studies of localisation and pathway modification by using the DARPins as intrabodies in WP5 and 6.