The department of Biochemistry of Kassel University focuses on in-depth interaction studies employing numerous biophysical and genetic techniques summarised as Biomolecular Interaction Analysis (BIA). In addition, the group is part of the Center of Interdisciplinary Nanostructure and Science (CINSaT) generating miniaturised devices for advanced biosensing at increased sensitivity and throughput.
The core expertise of the Biochemistry Department lies in functional proteomics based on detailed analysis of protein interactions. In addition to Biacore technology for high resolution kinetic analysis of binders (WP3), a number of technologies are established for increased throughput, including bead based technologies, such as AlphaScreen, and fluorescence based methods (Fluorescence Polarisation). Semi-automated in cell assays are in place to monitor protein-protein interactions, protein modifications and second messenger generation in living cells using a microtiter plate-based assay format.
Role in AFFINOMICS: For WP5,6, UNIKASSEL designs and implements protocols for the intracellular action of binders against kinases based on Bioluminescence Resonance Energy Transfer (BRET) and other techniques to monitor functional interference in signal transduction pathways.