Helmholtz Centre Munich
The HMGU (formerly the German Research Center for Environmental Health) is a large federal research centre focused on medicine and health care as well as ecosystems, which are of critical importance for health. The Department of Protein Science (Head, Marius Ueffing) is fully equipped to perform high throughput genomics and proteomics research as well as molecular and cellular biology. Research in the department focuses on disease-gene-identification, gene variance and the study of the pathogenetic mechanisms underlying inherited diseases.
Dr Marius Ueffing heads the Proteome Core Facility within the Gene Analysis Center. The HMGU antibody platform headed by Dr Elisabeth Kremmer has produced more than 1000 sets of rat and mouse monoclonal antibodies, of which a major fraction has been either published or licensed for commercial distribution. Both laboratories have access to the HMGU mouse facility and mouse clinic, one of Europe’s biggest animal facilities.
Role in AFFINOMICS: HMGU shares with EMBL MACF and Paratopes the production of monoclonal antibodies in WP2, from immunogen preparation, through immunisation and hybridomas to mAb scale-up, purification and labelling. In WP2, mice are immunised with consortium target peptides / proteins to derive hybridomas and sets of mAbs, for QC and validation (WP3) and applications (WP6). Between 5-10 antibodies will be raised against each target, generating sets of antibodies suited for different applications. The HMGU also focuses on setting up standards and SOPs for mAb binders. In WP5 they define standard requirements and methodological conditions for immunoprecipitation capture assays and coordinate WP6, where they carry out characterisation of protein complexes using immunoprecipitation and mass spectrometry.