WP1: Generation of protein and peptide antigens

Availability of antigens is a central requirement of the binder production pipeline and quality control tests. Antigens corresponding to about 1000 protein targets in the groups listed in Section 1.1 will be produced, prioritised by disease association, feasibility and current non-availability of validated binders. (Prioritisation algorithms according to known gene functions and cancer association will be contributed by WP7.) Diversity of antigen is needed for production of reagents recognising protein targets in native or denatured states and with sequence specific modification (phosphorylation). Three antigen types will be generated: recombinant folded domains or full length proteins generated in bacteria or eukaryotic cells (SGC, CPR); recombinant Protein Epitope Signature Tags (PrESTs), based on regions with low identity to other human proteins (KTH); and synthetic peptides, including phosphotyrosine modified sequences (DKFZ). At least two separate, non-overlapping epitopes (recombinant protein fragments or peptides) for each protein will be generated. We expect to produce all c. 1000 targets as linear fragments and about 500 as folded proteins or protein domains.