WP4: Next-generation selection systems for recombinant protein binders
The aim is to develop new and improved automated high throughput selection systems allowing multiple recombinant binders to be generated for each target, with ultimate capacity to create renewable affinity reagents to all human proteins. Streamlined robotic protocols based on novel concepts will be introduced, ultimately scalable for 50-100 targets per 6 week period. Highly parallel selection methods will include bead arrays, macroarrays and acoustic microfluidic chips. Technologies will encompass ribosome display for antibody fragments and DARPins (BBT, UZH), bacterial display for Affibody molecules (KTH), and phage display for antibody fragments from naïve, synthetic libraries (ULUND, TUBS). Resulting systems will be deployed during in WP2.